Protective Effect of Melatonin On Kainic acid Induced-Liver Damage And Immune Modulatory Cytokines
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Date
2014
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Publisher
Immunome Res.
Abstract
Liver toxicity occurs when liver develops inflammation due to exposure to several toxic substances. The study
was aimed to assess the liver damage induced by kainic acid and subsequently protective role of exogenous
melatonin against the liver toxicity. Interestingly, kainic acid caused severe liver damage as evident from deleterious
alterations in liver histology, increased lipid peroxide levels, decrease in the activities of liver anti-oxidant enzymes,
DNA damage (adduct formation and sequence alterations), increased expression of cytokines like monocyte chemoattractant
protein-1, interleukin 6, interferon γ and decreased expression of interleukin 10. These changes were
normalized by melatonin (0.5-1.0 mM, (in vitro) or 10–20 mg/kg, (in vivo)). The study included assessment of the
expression of immune modulatory cytokine mediators using real time PCR in both in vitro and in vivo conditions in
the mouse liver. DNA damage was also studied. Various oxidative stress parameters and liver histopathology was
also evaluated. Melatonin’s anti-kainic acid toxicity could be brought about by counter acting the influence of kainic
acid on the levels of the cytokines, immune reactions and free radical production. This work suggests that melatonin
receptors present could be mediate the hepatoprotective actions of melatonin therapy.
Description
Keywords
Melatonin; Kainic Acid; Liver Damage; Immune Modulation; Cytokines
Citation
Immunome Res.. 2014;S:2:-