Naked Eye Detection of Glucose in Urine Using Glucose Oxidase Immobilized Gold Nanoparticles

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dc.contributorRadhakumary, Changerath
dc.contributorSreenivasan, Kunnatheeri
dc.date.accessioned2012-12-04T11:44:41Z
dc.date.available2012-12-04T11:44:41Z
dc.date.issued2011
dc.description.abstractColloidal gold is extensively used for molecular sensing because of the flexibilities it offers in terms of modification of the gold nanoparticle surface with a variety of functional groups using thiol chemistry. We describe a simple assay that allows the visual detection of glucose in aqueous samples and demonstrates its applicability by estimating glucose in urine. To enable the glucose detection, we functionalized the thiol capped gold nanoparticles with glucose oxidase, the enzyme specific to beta-D glucose, using carbodiimide chemistry. The visible color change of the GOD-functionalized gold nanoparticles from red to blue on interaction with glucose is the principle applied here for the sensing of urine glucose level. The solution turns blue when the glucose concentration exceeds 100 mu g/mL. The approach depicted here seems to be important, particularly in third world countries where high tech diagnostics aids are inaccessible to the bulk of the population.
dc.identifier.citationANALYTICAL CHEMISTRY. 83; 7; 2829-2833en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac1032879
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/21391552
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/777
dc.publisherANALYTICAL CHEMISTRY
dc.subjectLaboratory Diagnosis
dc.titleNaked Eye Detection of Glucose in Urine Using Glucose Oxidase Immobilized Gold Nanoparticles
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