Matrix-directed differentiation of human adipose derived mesenchymal stem cells to dermal-like fibroblasts that produce extracellular matrix

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dc.contributor.authorUnnikrishnan, S
dc.contributor.authorJayakumar, K
dc.contributor.authorKrishnan, LK
dc.date.accessioned2015-06-02T06:49:42Z
dc.date.available2015-06-02T06:49:42Z
dc.date.issued2015-04
dc.description.abstractCommercially available skin substitutes lack essential non-immune cells for adequate tissue regeneration of non-healing wounds. A tissue-engineered, patient-specific, dermal substitute could be an attractive option for regenerating chronic wounds, for which adipose-derived mesenchymal stem cells (ADMSCs) could become an autologous source. However, ADMSCs are multipotent in nature and may differentiate into adipocytes, osteocytes and chondrocytes in vitro, and may develop into undesirable tissues upon transplantation. Therefore, ADMSCs committed to the fibroblast lineage could be a better option for in vitro or in vivo skin tissue engineering. The objective of this study was to standardize in vitro culture conditions for ADMSCs differentiation into dermal-like fibroblasts which can synthesize extracellular matrix (ECM) proteins. Biomimetic matrix composite, deposited on tissue culture polystyrene (TCPS), and differentiationmedium(DM), supplemented with fibroblast-conditioned medium and growth factors,were used as a fibroblast-specific niche (FSN) for cell culture. For controls, ADMSCswere cultured on bare TCPS with either DM or basal medium (BM). Culture of ADMSCs on FSN upregulated the expression of differentiation markers such as fibroblast-specific protein-1 (FSP-1) and a panel of ECM molecules specific to the dermis, such as fibrillin-1, collagen I, collagen IV and elastin. Immunostaining showed the deposition of dermal-specific ECM,which was significantly higher in FSN compared to control. Fibroblasts derived from ADMSCs can synthesize elastin, which is an added advantage for successful skin tissue engineering as compared to fibroblasts fromskin biopsy. To obtain rapid differentiation of ADMSCs to dermal-like fibroblasts for regenerative medicine, a matrix-directed differentiation strategy may be employed. Copyright © 2014 John Wiley & Sons, Ltden_US
dc.identifier.citationUnnikrishnan S, Jayakumar K, Krishnan LK. Matrix-directed differentiation of human adipose derived mesenchymal stem cells to dermal-like fibroblasts that produce extracellular matrix. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE. 2014en_US
dc.identifier.urihttp://dx.doi.org/10.1002/term.1865
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/2421
dc.publisherJOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINEen_US
dc.subjectextracellular matrix; autologous stem cells; adipose-derived mesenchymal stem cells; skin tissue engineering; dermal tissue regeneration; chronic wounds; differentiation; dermal fibroblastsen_US
dc.titleMatrix-directed differentiation of human adipose derived mesenchymal stem cells to dermal-like fibroblasts that produce extracellular matrixen_US
dc.typeArticleen_US
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