RAPID ISOLATION OF HUMAN PLASMA ANTI-ALPHA-GALACTOSIDE ANTIBODY USING SUGAR-SPECIFIC BINDING TO GUAR GALACTOMANNAN OR AGAROSE
| dc.contributor | JAISON, PL | |
| dc.contributor | APPUKUTTAN, PS | |
| dc.date.accessioned | 2012-12-04T11:45:03Z | |
| dc.date.available | 2012-12-04T11:45:03Z | |
| dc.date.issued | 1992 | |
| dc.description.abstract | A method of purifying the naturally occurring antibody to alpha-galactoside moiety (anti-alpha-Gal) in human plasma by a single-step affinity chromatography on cross-linked guar galactomannan (CLGG) or agarose (Sepharose 4B) is described. IgG nature of the two preparations, as revealed by agar gel diffusion, as well as their preference for alpha-anomer of galactose, as revealed in inhibition of their agglutination of trypsinized rabbit erythrocytes by sugars, identified them with anti-alpha-Gal. The antibody binding capacity of Sepharose 4B was only a third of that of CLGG. Both gels showed similar dependence on ionic strength for binding. The pH optimum for binding of anti-alpha-Gal to CLGG was 8.0. Significantly anti-alpha-Gal binding to Sepharose was unaffected by CNBr activation and ligand coupling to the gel, thus warning that contaminating plasma could introduce artifacts in agarose-based chromatography of human tissue biomolecules. | |
| dc.identifier.citation | INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS. 29; 3; 266-270 | en_US |
| dc.identifier.uri | https://dspace.sctimst.ac.in/handle/123456789/967 | |
| dc.publisher | INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | |
| dc.subject | Biochemistry | |
| dc.title | RAPID ISOLATION OF HUMAN PLASMA ANTI-ALPHA-GALACTOSIDE ANTIBODY USING SUGAR-SPECIFIC BINDING TO GUAR GALACTOMANNAN OR AGAROSE |