The antithrombotic and antimicrobial properties of PEG-protected silver nanoparticle coated surfaces

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dc.contributorRagaseema, V. M.
dc.contributorUnnikrishnan, S.
dc.contributorKrishnan, V. Kalliyana
dc.contributorKrishnan, Lissy K.
dc.date.accessioned2012-12-04T11:45:30Z
dc.date.available2012-12-04T11:45:30Z
dc.date.issued2012
dc.description.abstractCardiovascular implant-associated complications such as infection and thrombosis may be reduced by modification of device surfaces using antimicrobial and antithrombotic agents. Silver nanoparticles (SNPs) are well accepted for its broad-spectrum antimicrobial effect. A recent report suggested its antiplatelet effect also. So the hypothesis of this study is that polyethylene glycol (PEG) protected SNPs can be incorporated with biomaterials to attain dual properties; and by adjusting an optimum concentration, its cytotoxicity to tissues and cells can be prevented. To prove this, detailed study of PEG-SNP was done at three levels: (i) direct inhibitory effect on platelet activation, aggregation and biochemical pathways when PEG-SNP is added into platelet suspension; (ii) inhibition of platelet adhesion to PEG-SNP incorporated biological matrix and polymer scaffold and (iii) non-cytotoxic behavior of immobilized PEG-SNP in fibrin matrix. Inhibitory effects demonstrated are on: platelet function by aggregometry, exposure of activation and apoptosis markers by flow cytometry, biochemical pathway by malondealdehyde (MDA) estimation and protein phosphorylation by Western blot. Reduced platelet adhesion onto PEG-SNP incorporated scaffold is shown using scanning electron microscopy (SEM). Non-toxic behavior of endothelial cells (EC) and smooth muscle cells (SMC) grown on PEG-SNP-fibrin disc is shown by fluorescence microscopy and cell phenotype stability by real-time polymerase chain reaction (PCR). (c) 2012 Elsevier Ltd. All rights reserved.
dc.identifier.citationBIOMATERIALS. 33; 11; 3083-3092en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.biomaterials.2012.01.005
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/1186
dc.publisherBIOMATERIALS
dc.subjectBiocompatibility
dc.titleThe antithrombotic and antimicrobial properties of PEG-protected silver nanoparticle coated surfaces
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