Biomaterial properties of cholecyst-derived scaffold recovered by a non-detergent/enzymatic method

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dc.contributor.authorAnilkumar, TV
dc.contributor.authorVineetha, VP
dc.contributor.authorRevi, D
dc.contributor.authorMuhamed, J
dc.contributor.authorRajan, A
dc.date.accessioned2017-03-10T03:25:41Z
dc.date.available2017-03-10T03:25:41Z
dc.date.issued2014
dc.description.abstractIsolation procedures for the recovery of extracellular matrices (ECMs) from animal organs/tissues that are useful in regenerative medicine involve multiple sequential steps/stages including collection of the source organ at slaughter, their transportation to laboratory, decellularization, decontamination, stabilization, and sterilization. Most of these steps require extensive use of chemicals/reagents/enzymes which may also adversely affect the quality of the scaffold. With an effort to minimize the use of chemicals/reagents/enzymes, while extracting biomaterial-grade ECM from porcine cholecyst (gall bladder), we performed preisolation ex situ incubation of the organ in a stabilizing agent that also caused in situ crosslinking of tissue-components and delaminated the collagen-rich ECM from the tissue-layer beneath the mucosa. The physical, chemical, and biological properties of the isolated scaffolds were similar to that of a commercially available porcine small intestinal submucosa. The cholecyst-derived scaffold not only satisfied preclinical safety-test procedures such as cytotoxicity, local response, and endotoxin load but also showed the potential to promote healing of full-thickness skin wound in a rabbit model. The procedure was also suitable for isolating scaffolds from other hollow organs such as jejunum and urinary bladder. It was concluded that enzyme/detergent treatment may be an avoidable step while isolating biomaterial-grade scaffolds from hollow organs. (C) 2014 Wiley Periodicals, Inc.
dc.identifier.citation102 ,7;1506-1516en_US
dc.identifier.uri10.1002/jbm.b.33131
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/9372
dc.publisherJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
dc.subjectEngineering; Materials Science
dc.titleBiomaterial properties of cholecyst-derived scaffold recovered by a non-detergent/enzymatic method
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