A dot-immunobinding assay (dot-Iba) for rapid diagnosis of pulmonary tuberculosis.

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dc.contributorSumi, M G
dc.contributorMathai, A
dc.contributorReuben, S
dc.contributorRadhakrishnan, V V
dc.contributorSasikumar, S
dc.contributorJayapal, V
dc.contributorFelix, J
dc.date.accessioned2012-12-04T11:43:11Z
dc.date.available2012-12-04T11:43:11Z
dc.date.issued2001
dc.description.abstractIgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.
dc.identifier.citationIndian journal of experimental biology. 39; 10; 984-8en_US
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/11883521
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/16
dc.publisherIndian journal of experimental biology
dc.subjectLaboratory Diagnosis
dc.titleA dot-immunobinding assay (dot-Iba) for rapid diagnosis of pulmonary tuberculosis.
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