Strontium Hydroxyapatite scaffolds engineered with stem cells aid osteointegration and osteogenesis in osteoporotic sheep model.
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Date
2018-02
Journal Title
Journal ISSN
Volume Title
Publisher
Biointerfaces
Abstract
Osteoporotic fracture healing is an orthopaedic challenge due to excessive bone resorption and impaired
osteogenesis. Majority of currenttreatment strategies focus on regulating bone resorption and the potential
application of Mesenchymal Stem Cells (MSCs) in promoting osteogenesis has not been explored
much. Furthermore, the present study has put forth a novel approach, wherein the synergistic action of
Strontium (Sr) and MSCs in a single implant may facilitate osteoporotic bone healing.
Strontium Hydroxyapatite (SrHA) synthesized by wet precipitation was fabricated into tissue engineered
Strontium incorporated Hydroxyapatite (cSrHA) using sheep adipose tissue derived MSCs
(ADMSCs). Porosity, radiopacity and cytocompatibility of SrHA scaffolds were found appropriate for
orthopaedic applications. cSrHA scaffolds exhibited an in vitro Alkaline Phosphatase activity of 20 mol
pnp/30 min comparable to that of Hydroxyapatite (HA) – control scaffold, proving its osteogenic efficacy.
Implantation studies in sheep osteoporotic model depicted enhanced osteogenic ability with mature
lamellar bone formation in cSrHA implanted group, compared to bare HA, SrHA and tissue engineered HA
implanted groups. Histomorphometry data substantiated improved osteogenesis on par with material
resorption, as cSrHA implanted group exhibited highest regeneration ratio of 0.38 ± 0.05. Density histograms
from micro CT further signified the enhanced osteointegrative ability of cSrHA implants. Results
of the study depicted the therapeutic potential of cSrHA in osteoporotic bone healing and proposes the
use of allogenic ADMSCs for fabricating “Off the Shelf Tissue Engineered Products”
Description
Keywords
Osteoporosis Tissue engineering Strontium Hydroxyapatite Mesenchymal stem cells Osteoporotic sheep
Citation
Chandran S, Shenoy SJ, Babu S S, Nair RP, Varma H K , John A. Strontium Hydroxyapatite scaffolds engineered with stem cells aid osteointegration and osteogenesis in osteoporotic sheep model. Colloids and Surfaces B: Biointerfaces. 2018; 163:346-54.