Thiol functionalized polymethacrylic acid-based hydrogel microparticles for oral insulin delivery

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dc.contributorSajeesh, S.
dc.contributorVauthier, C.
dc.contributorGueutin, C.
dc.contributorPonchel, G.
dc.contributorSharma, Chandra P.
dc.date.accessioned2012-12-04T11:45:33Z
dc.date.available2012-12-04T11:45:33Z
dc.date.issued2010
dc.description.abstractIn the present study thiol functionalized polymethacrylic acid-polyethylene glycol-chitosan (PCP)-based hydrogel microparticles were utilized to develop an oral insulin delivery system Thiol modification was achieved by grafting cysteine to the activated surface carboxyl groups of PCP hydrogels (Cys-PCP). Swelling and insulin loading/release experiments were conducted on these particles The ability of these particles to inhibit protease enzymes was evaluated under in vitro experimental conditions Insulin transport experiments were performed on Caco-2 cell monolayers and excised intestinal tissue with an Ussing chamber set-up Finally, the efficacy of insulin-loaded particles in reducing the blood glucose level in streptozotocin-induced diabetic rats was investigated Thiolated hydrogel microparticles showed less swelling and had a lower insulin encapsulation efficiency as compared with unmodified PCP particles PCP and Cys-PCP microparticles were able to inhibit protease enzymes under in vitro conditions. Thiolation was an effective strategy to improve insulin absorption across Caco-2 cell monolayers, however, the effect was reduced in the experiments using excised rat intestinal tissue Nevertheless, functionalized microparticles were more effective in eliciting a pharmacological response in diabetic animal, as compared with unmodified PCP microparticles From these studies thiolation of hydrogel microparticles seems to be a promising approach to Improve oral delivery of proteins/peptides (C) 2010 Acta Materialia Inc Published by Elsevier Ltd All rights reserved
dc.identifier.citationACTA BIOMATERIALIA. 6; 8; 3072-3080en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.actbio.2010.02.007
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/20144748
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/1210
dc.publisherACTA BIOMATERIALIA
dc.subjectDrug Delivery
dc.titleThiol functionalized polymethacrylic acid-based hydrogel microparticles for oral insulin delivery
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