Zinc oxide nanoparticles mediated cytotoxicity, mitochondrial membrane potential and level of antioxidants in presence of melatonin
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Date
2017-06
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International Journal of Biological Macromolecules
Abstract
Zinc oxide nanoparticles (ZnO NPs) are widely used in a variety of products and are currently being investigated
for biomedical applications. However, they have the potential to interact with macromolecules
like proteins, lipids and DNA within the cells which makes the safe biomedical application difficult. The
toxicity of the ZnO NP is mainly attributed reactive oxygen species (ROS) generation. Different strategies
like iron doping, polymer coating and external supply of antioxidants have been evaluated to minimize
the toxic potential of ZnO NPs. Melatonin is a hormone secreted by the pineal gland with great
antioxidant properties. The melatonin is known to protect cells from ROS inducing external agents like
lipopolysaccharides. In the present study,the protective effect of melatonin on ZnO NPs mediated toxicity
was evaluated using C6 glial cells. The Cytotoxicity, mitochondrial membrane potential and free radical
formation were measured to study the effect of melatonin. Antioxidant assays were done on mice brain
slices, incubated with melatonin and ZnO NPs. The results of the study reveal that, instead of imparting
a protective effect, the melatonin pre-treatment enhanced the toxicity of ZnO NPs. Melatonin increased
antioxidant enzymes in brain slices.
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Keywords
ZnO NPs, Melatonin, ROS, Glial cells, Antioxidant molecules
Citation
Sruthi S, Mohanan PV. Zinc oxide nanoparticles mediated cytotoxicity, mitochondrial membrane potential and level of antioxidants in presence of melatonin. International Journal of Biological Macromolecules. 2017;103:808-18.