Assessment of Immunotoxicity of Dextran Coated Ferrite Nanoparticles in Albino Mice
No Thumbnail Available
Date
2015-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Molecular Biology International
Abstract
In this study, dextran coated ferrite nanoparticles (DFNPs) of size <25 nm were synthesized, characterized, and evaluated for
cytotoxicity, immunotoxicity, and oxidative stress by in vitro and in vivo methods. Cytotoxicity was performed in vitro using
splenocytes with different concentrations of DFNPs. Gene expression of selected cytokines (IL-1, IL-10, and TNF 𝛽) secretion by
splenocytes was evaluated. Also, 100 mg of DFNPs was injected intraperitoneally to 18 albino mice for immunological stimulations.
Six animals each were sacrificed at the end of 7, 14, and 21 days. Spleen was subjected to immunotoxic response and liver was analyzed
for antioxidant parameters (lipid peroxidation, reduced glutathione, glutathione peroxidase, superoxide dismutase, and glutathione
reductase). The results indicated that DFNPs failed to induce any immunological reactions and no significant alternation in
antioxidant defense mechanism. Also, mRNA expression of the cytokines revealed an increase in IL-10 expression and subsequent
decreased expression of IL-1 and TNF 𝛽. Eventually, DNA sequencing of liver actin gene revealed base alteration in nonconserved
regions (10–20 bases) of all the treated groups when compared to control samples. Hence, it can be concluded that the DFNPs were
nontoxic at the cellular level and nonimmunotoxic when exposed intraperitoneally to mice.
Description
Keywords
Citation
Syama S, Gayathri V, Mohanan PV. Assessment of Immunotoxicity of Dextran Coated Ferrite Nanoparticles in Albino Mice. Molecular Biology International. 2015.