A Simple Economical Method for Assay of Atherogenic Small Dense Low-Density Lipoprotein-Cholesterol (sdLDL-C).
dc.contributor | Renjith, R S | |
dc.contributor | Jayakumari, N | |
dc.date.accessioned | 2012-12-04T11:43:14Z | |
dc.date.available | 2012-12-04T11:43:14Z | |
dc.date.issued | 2011 | |
dc.description.abstract | In the present study, we report a simple and economical precipitation method for the quantitative determination of small, dense LDL-cholesterol (sdLDL-C) in serum that is considered to be an emerging risk factor for cardiovascular disease. This method consisted of precipitation of lipoproteins of density <1.044g/ml using heparin-MnCl(2) and quantification of cholesterol existed in the supernatant using reagents for routine cholesterol assay instead of the costly direct low density lipoprotein-cholesterol assay kit. The supernatant contained sdLDL and high-density lipoprotein (HDL) that was confirmed by polyacrylamide gel electrophoresis. sdLDL-C concentration can be calculated by subtracting the HDL-C value from the total cholesterol concentration of the supernatant. sdLDL-C values obtained by this modified method were similar to those obtained by direct assay of sdLDL-C and there was significant correlation between the two methods. In conclusion, this method is highly economical, do not require special equipments and is useful to evaluate atherogenic risk. | |
dc.identifier.citation | Indian journal of clinical biochemistry : IJCB. 26; 4; 385-8 | en_US |
dc.identifier.uri | https://dspace.sctimst.ac.in/handle/123456789/38 | |
dc.publisher | Indian journal of clinical biochemistry : IJCB | |
dc.subject | Biochemistry | |
dc.title | A Simple Economical Method for Assay of Atherogenic Small Dense Low-Density Lipoprotein-Cholesterol (sdLDL-C). |