RAPID DIAGNOSIS OF TUBERCULOUS MENINGITIS WITH A DOT ENZYME-IMMUNOASSAY TO DETECT ANTIBODY IN CEREBROSPINAL-FLUID

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dc.contributorMATHAI, A
dc.contributorRADHAKRISHNAN, VV
dc.contributorTHOMAS, M
dc.date.accessioned2012-12-04T11:45:03Z
dc.date.available2012-12-04T11:45:03Z
dc.date.issued1991
dc.description.abstractA simple dot enzyme immunoassay (Dot-EIA) was carried out to detect antibody to Mycobacterium tuberculosis antigen 5 in cerebrospinal fluid (CSF) specimens from 40 patients with a clinical diagnosis of tuberculous meningitis (TBM). The assay gave a positive reaction in all ten patients with culture proven TBM. In 30 culture negative patients with TBM, the assay was positive at a titre of 1:16 in 18 patients. In 40 patients with non-tuberculous neurological diseases (control group) the assay was negative at a titre of 1:16. The Dot-EIA had an overall sensitivity of 70% and a specificity of 100% in the diagnosis of TBM. This assay could be used as a rapid screening test to establish the diagnosis of TBM, particularly in patients in whom bacteriological investigations for Mycobacterium tuberculosis in CSF specimens are negative.
dc.identifier.citationEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. 10; 5; 440-443en_US
dc.identifier.urihttp://dx.doi.org/10.1007/BF01968025
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/966
dc.publisherEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
dc.subjectImmunology
dc.titleRAPID DIAGNOSIS OF TUBERCULOUS MENINGITIS WITH A DOT ENZYME-IMMUNOASSAY TO DETECT ANTIBODY IN CEREBROSPINAL-FLUID
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