Induction of immunotoxicity and oxidative stress by imidazole on immune cells
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Date
2015-07
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Applied Cell Biology
Abstract
Imidazole is a known irritant to rabbit eye and possesses moderate oral
toxicity. However Imidazole toxicity to immune cells if any, is unclear
therefore needs to be scientifically evaluated. Aim of the present study
was to evaluate the immuno-toxicity properties, if any, of imidazole par- ent compound. The study included assessment of the expression of im- mune modulatory cytokine mediators using real time PCR from the total
RNA, isolated from mixed and T lymphocytes. Nitrosative stress was
assessed by Nitric oxide formation. Apoptotic potential of imidazole on
T lymphocytes using Annexin V assay kit was carried out using flow cytometer.
Higher concentration of imidazole (10mg/ml) showed a sig- nificant increase in pro inflammatory cytokines like interleukin-1, mono- cyte chemo attractant protein-1, tumour necrosis factor beta, whereas
drastic reduction in anti inflammatory cytokine, interleukin -10 was noted. This study also demonstrated that Imidazole (10 mg/ml) induces mixed
and T lymphocyte proliferation by DNAsynthesissignificantly. The mRNA
expressions of mitogen-activated protein kinases 14 gene, inducible Nitric
oxide synthase and Bcl-2ñassociated X protein were also signifi- cantly increased (p<0.01). However, lower concentrations of imidazole
did not exhibit such effect on both types of cells. Continuous exposure
of workers to imidazole used in industries could suppress the bodyís
immune system, especially cellular immunity thereby triggering the inflammatory
pathway and targeting the p38 MAPK signal transduction
pathway. These observations are of interest in view of workers in pesti- cide, pharmaceutical industries
Description
Keywords
Imidazole; Splenocytes; T lymphocytes; Cytokines; Oxidative stress; Apoptosis.
Citation
Gayathri V, Mohanan PV. Induction of immunotoxicity and oxidative stress by imidazole on immune cells. Applied Cell Biology. 2015;4(2):45-55