Preparation of hydroxyapatite porous scaffolds from a coral like synthetic inorganic precusrsor for use as a bone graft substitute and a drug delivery vehicle

Nimi, N; Palangadan, R; Fernandez, FB; Varma, H

Preparation of hydroxyapatite porous scaffolds from a coral like synthetic inorganic precusrsor for use as a bone graft substitute and a drug delivery vehicle

Date

2018-11

Authors

Publisher

Materials Science and Engineering C

Abstract

A novel surfactant free hydrothermal method was developed for the preparation of large hydroxyapatite scaffolds. Synthetic calcium carbonate (calcite) was used as the starting material which when mixed with an inorganic setting solution containing phosphoric acid and sodium hydroxide forms the porous precursor body with pore size 20-700 μm. The porous precursor body was then hydrothermally converted to hydroxyapatite scaffolds when treated in basic phosphate solution of pH 10.5 at 150 °C and 15 bar pressure maintaining the structural stability and integrity. X-ray diffraction and the Fourier transform infrared spectroscopy confirmed that the developed material consist of single phase crystalline hydroxyapatite. Surface morphology and microstructures were studied using scanning electron microscopy and porosity was evaluated by micro CT analysis. The cell material interactions evaluated by cell viability assays and live cell staining methods confirmed the cell compatibility. The drug release study at physiological pH implied that the developed materials could be promising in sustained long-term release. The results emerged have shown that the hydrothermal conversion of inorganic coral-like precursor is effective to produce porous bioactive hydroxyapatite scaffolds for bone regeneration as well as drug delivery vehicles for the treatment of infectious bone diseases such as osteomyelitis.

Keywords

Bone substitute; Calcite; Hydrothermal; Hydroxyapatite; Porous scaffold

Citation

Nimi N, Palangadan R, Fernandez FB, Varma H. Preparation of hydroxyapatite porous scaffolds from a coral like synthetic inorganic precusrsor for use as a bone graft substitute and a drug delivery vehicle. Materials Science and Engineering C. 2018 Nov ; 92:329- 337.