Negative inotropic response to cerium in ventricular papillary muscle is mediated by reactive oxygen species
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Date
2003
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Publisher
BIOLOGICAL TRACE ELEMENT RESEARCH
Abstract
This study was performed with the objective of assessing the mechanical response of the myocardium. to different levels of cerium and delineation of the mechanism underlying the mediation of the functional changes. Rat ventricular papillary muscle was used as the experimental model. Isolated papillary muscles were exposed to different concentrations of CeCl3 and the force of contraction was measured using a force transducer. Experiments have revealed that the negative inotropic response to CeCl3 was proportional to its concentration. The inotropic changes were found to be completely reversible at concentrations less than or equal to 5 muW, and partially reversible at higher concentrations. Neutralization of cerium-induced inotropic changes by the superoxide anion scavenger superoxide dismutase (SOD) at concentrations less than or equal to 5 muW indicates that the mechanical changes are mediated by reactive oxygen species. At higher concentrations of Ce3+, SOD partially reversed the contractile changes. The beneficial effect of SOD was seen only if the muscles were pretreated with the scavenger prior to the addition of cerium chloride.
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Keywords
Cardiology
Citation
BIOLOGICAL TRACE ELEMENT RESEARCH. 96; 40911; 203-208