Pullulan-protamine as efficient haemocompatible gene delivery vector: Synthesis and in vitro characterization

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dc.contributor.authorPriya, SS
dc.contributor.authorRekha, MR
dc.contributor.authorSharma, CP
dc.date.accessioned2015-01-08T07:03:23Z
dc.date.available2015-01-08T07:03:23Z
dc.date.issued2014-02
dc.description.abstractBiodegradable non-viral vectors with good transfection efficiency is essential for successful gene delivery. The purpose of this study was to design a non-viral vector by conjugating protamine to pullulan and elucidate the potential use of pullulan protamine conjugate (PPA) as an effective, non toxic and haemocompatible gene delivery system. The particle size and surface charge were measured using Nanosizer. Derivatization was confirmed by NMR, FTIR and DSC analyses. Acid base titration revealed the buffering behaviour of the conjugate. The protection of DNA from nuclease enzyme and interaction of plasma components on the stability of nanoplexes were also analysed. The uptake studies confirmed the plasmid delivery into the nucleus and the inhibitor studies determined the uptake mechanism. Transfection experiments revealed the capability of PPA to cellular uptake in C6 cells and facilitate high gene expression. Thus, PPA proves to be a promising non-viral vector.en_US
dc.identifier.citationCarbohydrate Polymers. 2014;102(1):207-15en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.carbpol.2013.11.024
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/2283
dc.publisherCarbohydrate Polymers.en_US
dc.subjectCationized pullulan; Gene delivery; Haemocompatible; Non- viral vector; Protamineen_US
dc.titlePullulan-protamine as efficient haemocompatible gene delivery vector: Synthesis and in vitro characterizationen_US
dc.typeArticleen_US
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