In vitro characterization of differently processed decellularised bovine pericardium
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Date
2016-08
Journal Title
Journal ISSN
Volume Title
Publisher
J Livestock Science
Abstract
The efficacy of two different in vitro protocols for the decellularisation of bovine pericardium has been
evaluated in this experiment. The pericardia were decellularised using a non-detergent based proprietary
enzymatic protocol and a biodetergent (deoxycholic acid) based new protocol. The in vitro characterisation to
estimate the in vivo stability was done using histological characterisation, residual DNA analysis, uniaxial
tensile testing, contact angle measurement and collagenase susceptibility. The results indicated that the newr
detergent based protocol was equally effective in inducing decellularisation and produce similar in vitro
characteristics as in enzymatically processed pericardium, and hence can be used for in vivo evaluation and
experimental studies.
Description
Keywords
pericardium, bovine, decellularisation, in vitro characterisation
Citation
Suvaneeth P, Divakaran N N, Ramachandra U P, Narayanan N, Abraham M J, Divya C Martin J. In vitro characterization of differently processed decellularised bovine pericardium. J Livestock Science. 2015;7:13-18