Fluorescence spectroscopy as an efficient tool for staging the degree of liver fibrosis: an in vivo comparison with MRI
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Date
2018-07
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Sci Rep. 2018
Abstract
The study utilizes autofuorescence spectroscopy (AFS) along with multivariate spectral analysis
for diferentiating various stages of hepatic fbrosis. AFS has recently emerged as an efcient tool
for evaluating the variations in diferent endogenous furophores. In this study, the potential of AFS
for diferentiating the stages of liver fbrosis is assessed and compared with the results of enzyme
evaluation, histopathology and the most advanced diagnostic tool, MRI. Using a fber optic probe,
the emission profle of the furophores such as favin adenine dinucleotide (FAD), lipofuscin-like
lipopigments (lipopigments), porphyrins and the variation in the total hemoglobin concentration are
evaluated in vivo on liver fbrosis induced animal models adopting a minimally invasive technique.
Signifcant diference (p<0.05) in the level of these biomarkers was observed between diferent stages
of liver fbrosis. Normal hepatic tissue could be distinguished from mild and moderate hepatic fbrosis
with a sensitivity of 95 to 100% and specifcity of 90 to 100% using multivariate spectral analysis. The
results are favourable to consider this technique as a potential tool for diagnosing liver fbrosis at an
early stage, which is monumental as it otherwise can lead to cirrhosis and liver failure.
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Citation
Nazeer SS, Saraswathy A, Shenoy SJ, Jayasree RS. Fluorescence spectroscopy as an efficient tool for staging the degree of liver fibrosis: an in vivo comparison with MRI. Sci Rep. 2018 Jul 20;8(1): 10967.