Determination of antioxidant defense mechanism after acute oral administration of hydroxyapatite nanoparticles in rats.
No Thumbnail Available
Date
2014-03
Journal Title
Journal ISSN
Volume Title
Publisher
J Free Rad Antioxidants
Abstract
Hydroxyapatite nanoparticle (HANPs) has various
applications like bone grafting, dental fillings, in
toothpastes and mouth washes for teeth
remineralization, etc. Even though HANPs
application is a widely investigated area of
research, the toxicity aspect still remains largely
unexplored. This study focuses on the acute oral
toxicity of HANPs exposed to Wistar rats, as its
prospective application involve exposure via the
oral route. In-house synthesized HANPs (<50 nm)
was administered to rats orally. Hematological and
biochemical parameters were carried out in blood
from these animals. After sacrifice, gross necropsy
was conducted and lipid peroxidation (LPO) and
antioxidant status was estimated in the liver. During
the experimental period, no HANPs related toxicity
was observed and the hematological and
biochemical parameters remained similar to control.
However, dose dependent LPO in liver but the
resultant oxidative stress was combated by the cells
innate antioxidant defense mechanisms, which was
evident with the increase in activity. Even though no
death or adverse toxic effects were observed in the
acute oral toxicity (unclassified compound as per
Globally Harmonized System for Classification for
chemical substances and mixtures) according to
OECD 423, there was a dose dependant increase
in the oxidative stress.
Description
Keywords
Hydroxyapatite, nanoparticles, acute oral toxicity, oxidative stress, antioxidant enzyme haematology, biochemistry
Citation
J Free Rad Antioxidants.. 2014;140:318- 27