Detection of interleukin-1 from isolated human lymphocyte in response to lipopolysaccharide and lipoteichoic acid

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dc.contributor.authorLekshmi, N
dc.contributor.authorGeetha, CS
dc.contributor.authorMohanan, PV
dc.date.accessioned2017-03-10T03:26:11Z
dc.date.available2017-03-10T03:26:11Z
dc.date.issued2012
dc.description.abstractAim: To detect the interleukin -1 levels from single and pooled isolated human lymphocytes in response to lipolysaccharide and lipoteichoic acid. Materials and Methods: Blood collected from healthy individuals (O ve, A ve, B ve, and AB ve) were subjected to gradient centrifugation to isolate lymphocytes. Different lymphocyte concentrations were used for in vitro pyrogen assay. Lymphocytes isolated were challenged with 5 EU of Gram negative (LPS) and 1 g/l of Gram positive (LTA) pyrogens in vitro and the inflammatory cytokine, Interleukin 1 (IL-1) release was measured by Sandwich ELISA method. Results: The results indicated that the release of IL-1 increases immediately after the initiation of incubation and reaches a maximum at 4 to 6 (th) hour and then stabilizes for both the pyrogens. Furthermore, IL-1 release by 5 EU of LPS and 1 g/l of LTA is dependent on lymphocytes concentration. It was also observed that the difference in blood group did not interfere with the IL-1 release. Conclusion: The isolated lymphocyte system can be used as an alternative to the in vivo rabbit pyrogen assay.
dc.identifier.citation44 ,6;726-731en_US
dc.identifier.uri10.4103/0253-7613.103269
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/9561
dc.publisherINDIAN JOURNAL OF PHARMACOLOGY
dc.subjectPharmacology & Pharmacy
dc.titleDetection of interleukin-1 from isolated human lymphocyte in response to lipopolysaccharide and lipoteichoic acid
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