Characterization of mycobacterial antigens by Elisa and immunoblot methods.

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dc.contributorMathai, A
dc.contributorRadhakrishnan, V V
dc.contributorSehgal, S
dc.date.accessioned2012-12-04T11:43:37Z
dc.date.available2012-12-04T11:43:37Z
dc.date.issued1995
dc.description.abstractAntibodies to two mycobacterial antigens viz - culture filtrate antigen (CFA) and Mycobacterium tuberculosis antigen 5 were raised in rabbits. Enzyme-linked immuno sorbent assay (ELISA) and immunoblot methods were used for the evaluation of the specificity of the rabbit antibodies to M. tuberculosis. Immunoblot method is more sensitive than ELISA for the detection of antibodies to M. tuberculosis in the rabbit sera. It is being emphasised that characterisation of the mycobacterial antigens and evaluation of the specificity of the antimycobacterial antibodies are essential prior to their applications as an adjunct in the laboratory diagnosis of human mycobacterial disease.
dc.identifier.citationIndian journal of pathology & microbiology. 38; 3; 299-303en_US
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/242
dc.publisherIndian journal of pathology & microbiology
dc.subjectImmunology
dc.titleCharacterization of mycobacterial antigens by Elisa and immunoblot methods.
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