Betaine conjugated cationic pullulan as effective gene carrier

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dc.contributor.authorLizebona, AA
dc.contributor.authorRekha, MR
dc.date.accessioned2015-05-07T04:21:42Z
dc.date.available2015-05-07T04:21:42Z
dc.date.issued2015-02
dc.description.abstractPolyethyleneimne (PEI) is a very efficient transfecting agent but is toxic due to high charge density. To generate a vector which is efficient and less cytotoxic, PEI was conjugated with pullulan (PPEI). Further conjugation was done on PPEI with zwitter ionic betaine which possess antifouling property. PEI of molecular weight 1.2, 2, and 10 kDa were used in the study. Buffering capacity of pullulan-PEI-betaine (PPB) conjugates was found to be sufficient enough for the polymers to make endosomal escape. The polymers proved to be less cytotoxic and highly hemocompatible than PEI. Nuclear localization of YOYO tagged DNA was observed with the nanoplexes developed using PPEI and PPBs of PEI 10 kDa. Transfection efficiency was evaluated using p53 expressing gene and the live dead assay demonstrated very high transfection efficiency with PPB conjugates of PEI 10 kDaen_US
dc.identifier.citationLizebona AA, Rekha MR. Betaine conjugated cationic pullulan as effective gene carrier. International Journal of Biological Macromolecules. 2015;72:819-26en_US
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/25304750
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/2393
dc.publisherInternational Journal of Biological Macromoleculesen_US
dc.titleBetaine conjugated cationic pullulan as effective gene carrieren_US
dc.typeArticleen_US
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