A Near-Infrared Fluorescent Nanosensor (AuC@Urease) for the Selective Detection of Blood Urea
No Thumbnail Available
Date
2013-04
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Small
Abstract
The use of a nanosensor (AuC@Urease) based on an NIR-emitting gold cluster and urease enzyme selectively detects urea in whole blood. The detection is based on an enzyme-specific conversion of urea to ammonium ions which facilitates pH-induced aggregation of AuC, leading to fluorescence quenching. This method does not interfere with urease inactive analytes or the autofluorescence of blood as confirmed by comparable urea levels obtained by AuC@Urease and standard clinical methods within an error limit of ±3%
Description
Keywords
Citation
Small. 2013; DOI 10.1002/smll.201300213