EPITOPES RECOGNIZED BY SERUM ANTI-ALPHA-GALACTOSIDE ANTIBODY ARE PRESENT ON BRAIN GLYCOPROTEINS IN MAN

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dc.contributor.authorJAISON, PL
dc.contributor.authorKANNAN, VM
dc.contributor.authorGEETHA, M
dc.contributor.authorAPPUKUTTAN, PS
dc.date.accessioned2017-03-10T03:26:34Z
dc.date.available2017-03-10T03:26:34Z
dc.date.issued1993
dc.description.abstractNaturally occurring serum IgG against terminal alpha-galactoside epitopes (anti-Gal), present exclusively in man, apes and old world monkeys, was used as probe for these epitopes in human brain. Human brain grey matter soluble glycoproteins enriched in alpha-galactosyl groups by affinity chromatography on jacalin-sepharose, specifically binds to human anti-Gal in immuno dot blots. Anti-Gal recognized exclusively the terminal alpha-galactoside epitope in human brain glycoproteins since binding was abolished by the presence of 1-0-methyl alpha-D-galactopyranoside as well as by pretreatment of glycoproteins with coffee bean alpha-galactosidase. Anti-Gal-peroxidase staining of jacalin-binding human brain glycoproteins in western immuno blots revealed mainly five anti-Gal-binding polypeptides with M(r) (in kDa) of 94, 108, 180, 210 and 230 respectively. Since the presence of anti-Gal in higher animals accompanies suppression of the corresponding epitope in most tissues, apparently to maintain immunological balance, possible implications of the above observation for autoimmunity, tumor metastasis and infection are discussed.
dc.identifier.citation18 ,2;187-193en_US
dc.identifier.uri10.1007/BF02703115
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/9706
dc.publisherJOURNAL OF BIOSCIENCES
dc.subjectLife Sciences & Biomedicine - Other Topics
dc.titleEPITOPES RECOGNIZED BY SERUM ANTI-ALPHA-GALACTOSIDE ANTIBODY ARE PRESENT ON BRAIN GLYCOPROTEINS IN MAN
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