Characterization of Fibroin and PEG-Blended Fibroin Matrices for In Vitro Adhesion and Proliferation of Osteoblasts

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dc.contributor.authorAcharya, C
dc.contributor.authorKumary, TV
dc.contributor.authorGhosh, SK
dc.contributor.authorKundu, SC
dc.date.accessioned2017-03-10T03:25:53Z
dc.date.available2017-03-10T03:25:53Z
dc.date.issued2009
dc.description.abstractSilk fibroin protein, isolated from cocoons of the domesticated mulberry silkworm, Bombyx mori, finds extensive application in biomaterial design. In this study, poly(ethylene glycol) (PEG) 4000 has been used for blending fibroin from both B. mori and Antheraea mylitta, the wild tropical non-mulberry silkworm. PEG-blended films have shown marked changes from the pure fibroin films with respect to thermal properties and mechanical properties. FT-IR spectroscopy confirmed incorporation of new functional groups like quinone oximes. Pure fibroin and PEG-blended fibroin films showed biocompatibility with the HOS osteosarcoma cell line. Von Kossa staining confirmed nodule formation due to mineralization and differentiation of osteoblasts on pure and blended matrices. On account of increased surface roughness, higher elongation percentage, higher thermostability and better activity of osteoblasts in terms of intracellular alkaline phosphatase production, PEG-blended A. mylitta fibroin film shows better potential than PEG-blended B. mori fibroin film for use as potential biomaterial. (C) Koninklijke Brill NV, Leiden, 2009
dc.identifier.citation20 ,42891;543-565en_US
dc.identifier.uri10.1163/156856209X426385
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/9444
dc.publisherJOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
dc.subjectEngineering; Materials Science; Polymer Science
dc.titleCharacterization of Fibroin and PEG-Blended Fibroin Matrices for In Vitro Adhesion and Proliferation of Osteoblasts
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