Rapid diagnosis of tuberculous meningitis by a dot-immunobinding assay

Simple item page
dc.contributorSumi, MG
dc.contributorAnnamma, M
dc.contributorSarada, C
dc.contributorRadhakrishnan, VV
dc.date.accessioned2012-12-04T11:45:03Z
dc.date.available2012-12-04T11:45:03Z
dc.date.issued2000
dc.description.abstractIn this study, a dot-immunobinding assay (Dot-Iba) was standardized to measure circulating antimycobacterial antibodies in the cerebrospinal fluid (CSF) specimens for the rapid laboratory diagnosis of tuberculous meningitis (TBM). Specific CSF-IgG antibody to Mycobacterium tuberculosis from a culture proven patient with TBM was isolated and coupled with activated Cynogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen present in the culture filtrates of ill. tuberculosis was isolated and this antigen was used in the Dot-Iba, to quantitate specific antimycobacterial antibodies in CSF specimens. The Dot-Iba gave positive results in all the 5 culture proven patients with TBM and gave no false positive results in CSFs from patients with partially treated pyogenic meningitis. Dot-Iba developed in our laboratory is a simple, rapid and specific method and more importantly suited for the routine application in laboratories with limited resources.
dc.identifier.citationACTA NEUROLOGICA SCANDINAVICA. 101; 1; 61-64en_US
dc.identifier.urihttp://dx.doi.org/10.1034/j.1600-0404.2000.00012.x
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/10660155
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/965
dc.publisherACTA NEUROLOGICA SCANDINAVICA
dc.subjectImmunology
dc.titleRapid diagnosis of tuberculous meningitis by a dot-immunobinding assay
Files
Collections
Journal Articles