In vitro hematological and in vivo immunotoxicity assessment of dextran stabilized iron oxide nanoparticles
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Date
2015-07
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Colloids and Surfaces B: Biointerfaces
Abstract
Iron oxide nanoparticles have attracted enormous interest as potential therapeutic agents. The purpose
of this study was to examine the in vitro hematological toxicity and in vivo immune response toward previously
synthesized and characterized dextran stabilized iron oxide nanoparticles (DIONPs) developed
for hyperthermia application. Peripheral whole blood from human volunteers was used to investigate
hemolysis, platelet aggregation, lymphocyte proliferation and cytokine mRNA expression induced by
DIONPs in vitro. In the concentration range of 0.008–1 mg/ml, DIONPs did not induce relevant levels of
hemolysis or platelet aggregation. Assessment of lymphocyte function showed significant suppression of
the proliferation activity of T-lymphocytes in cultures stimulated with the mitogen phytohemagglutinin
(PHA). In addition, inhibition of PHA-induced cytokine mRNA expressions was also seen. However, systemic
administration of DIONPs resulted in enhanced proliferation of mitogen-stimulated spleen derived
lymphocytes and secretion of IL-1 at day 7 post exposure. In conclusion, our results demonstrate that
immune response is influenced variably by nanoparticles and its degradation milieu. Further investigation
of the observed immunosuppressive effects of DIONPs in immune stimulated animal models is
required to assess the functional impact of such a response.
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Keywords
Iron oxide nanoparticles Hemolysis Platelet aggregation Lymphocyte proliferation Cytokines Intravenous
Citation
Easo SL, Mohanan PV. In vitro hematological and in vivo immunotoxicity assessment of dextran stabilized iron oxide nanoparticles. Colloids and Surfaces B: Biointerfaces. 2015;134:122-30