Utility of interphase fluorescent in situ hybridization for diagnosis of brain tumors

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Brain tumours are classified as per the 5th edition of the WHO classification of tumours of the central nervous system. This latest edition of CNS tumors incorporates molecular features along with the conventional histology leading to “histomolecular” classification. Interphase Fluorescent in situ hybridization (iFISH) is a useful ancillary technique to the pathologist. It is useful to identify gene deletions, amplifications and rearrangements. Fluorescent-tagged FISH probes are hybridized to formalin-fixed paraffin-embedded sections on glass slides and the nuclei are observed under a fluorescent microscope. iFISH is particularly useful in a resource constrained setting where more advanced aids such as methylation profiling and targeted panels are not readily available. The iFISH technique performed in the pathology department will be discussed and the utility of iFISH in neuro-oncology diagnostics will be illustrated through a case-based discussion.