Self-assembled superparamagnetic nanocomposite-labelled cells for noninvasive, controlled, targeted delivery and therapy
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Date
2015-04
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RSC Advances
Abstract
Efficient delivery of cells to targeted sites at optimal concentrations within rational limits of damage to
normal tissue is a major challenge for cell delivery. With the help of magnetic nanoparticles binding to
the surface of cells, it is possible to manipulate and control cell mobility using an external magnetic field.
Here, we demonstrate physical entrapment of magnetic nanocomposites onto cell surfaces and their
manipulation by an external magnetic field. Uniformly embedded nano iron oxide particles in a
hydroxyapatite crystallite (HAIO) were synthesized via co-precipitation method. Physiochemical and
biological evaluation of the above nanocomposite system showed that the HAIO containing 50 wt% iron
oxide (HAIO50) possessed excellent magnetic properties and good cytocompatibility. Prussian blue
staining and flow cytometric evaluation of cell–material interactions indicated uniform uptake and a
dose-dependent interaction. HAIO50 is found to be a novel matrix for use as an effective and
cytocompatible avenue for cell separation, evidenced via Coulter analysis as well as fluorescent imaging
of live cells. Post-magnetic separation analysis of cell viability via confocal laser scanning microscopy
(CLSM) showed the normal structure and proliferation of separated cells. HAIO50 may be used as an
efficient matrix for magnetic non-invasive manipulation and for further cell delivery applications
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Citation
Beeran AE,Francis Fernandez FB, John A, Harikrishna Varma PR. Self-assembled superparamagnetic nanocomposite-labelled cells for noninvasive, controlled, targeted delivery and therapy. RSC Advances. 2015;5:36742-52.