Nano-bio compatibility of PEGylated reduced graphene oxide on mesenchymal stem cells.
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Date
2017-05
Journal Title
Journal ISSN
Volume Title
Publisher
2D Materials
Abstract
Graphene, with its unique physico-chemical properties, has found widespread biomedical
application. It is used as a carrier for drug or gene delivery, photothermal therapy, bioimaging, in
antibacterial agents and for the development of biosensors. Besides this, graphene has the scope
to be used for wound healing, tissue engineering and regenerative medicine. In the present study,
polyethylene-glycol-(PEG)ylated reduced graphene oxide (PrGO) was synthesized, characterized,
and its interaction with mouse bone marrow mesenchymal stem cells (MSCs) was studied. in vitro
cytotoxicity and differentiation study showed PrGO neither induced toxicity nor impaired the
differentiation potential of the stem cells. PrGO was effectively internalized by MSCs and distributed
throughout the cytoplasm. None of the PrGO was seen in the nucleus. Although it seems to induce
increased reactive oxygen species (ROS) production inside the cell, no change in cell proliferation
or cellular function was observed. Hence it is recommended that the synthesized PrGO is applicable
for tissue engineering, and can also be used as a substrate platform for stem cell culture and
differentiation.
Description
Keywords
PEGylated reduced graphene oxide, mesenchymal stem cells, cytotoxicity, apoptosis, reactive oxygen species, mitochondrial membrane potential
Citation
Syama S, Aby CP, Maekawa T, Sakthikumar D, Mohanan PV. Nano-bio compatibility of PEGylated reduced graphene oxide on mesenchymal stem cells. 2D Materials. 2017;4(2):25066.