. Exploring the Potential of Alginate-Gelatin-Diethylaminoethyl Cellulose Fibrinogen based Bioink for 3D Bioprinting of Skin

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dc.contributor.authorRamakrishnan, R
dc.contributor.authorKasoju, N
dc.contributor.authorRaju, R
dc.contributor.authorGeevarghese, R
dc.contributor.authorGauthaman, A
dc.contributor.authorBhatt, A
dc.date.accessioned2022-03-29T06:00:27Z
dc.date.available2022-03-29T06:00:27Z
dc.date.issued2022-06
dc.description.abstractDesigning printable bioinks for 3D bioprinting capable of supporting cellular viability with post-printing functionality remains challenging. Native ECM offers several physical, chemical, and biological cues that are difficult to restore using only a single component. Herein, we have optimized a multicomponent-based bioink formulation comprising alginate (ALG), gelatin (GEL), diethylaminoethyl cellulose (DCEL) and fibrinogen (FIB), termed as ALG-GEL-DCEL-FIB bioink for potential application in bioprinting and biofabrication of skin tissue equivalents. The designed formulation was extensively studied for its printability, physico-chemical, rheological, and biocompatibility properties. Excellent printability, shape fidelity and cell-laden tissue equivalent printing were established using the RegenHu 3D Discovery Bioprinter. The human primary fibroblast and keratinocyte-laden bioprinted constructs exhibited good cell viability. Long term culture of 4 weeks comprising 5 days of air-liquid-interphase followed by 21 days of submerged culture produced biomimetic tissue histology in the ALG-GEL-DCEL-FIB bioink printed constructs. Specific epidermal-dermal marker expressions proving functionality were evident in immunohistochemical, biochemical and gene expression analysis. The ALG-GEL-DCEL-FIB bioink may be explored further for potential biofabrication and therapeutic applications.en_US
dc.identifier.citationRamakrishnan R, Kasoju N, Raju R, Geevarghese R, Gauthaman A, Bhatt A. Exploring the Potential of Alginate-Gelatin-Diethylaminoethyl CelluloseFibrinogen based Bioink for 3D Bioprinting of Skin. Carbohydrate Polymer Technologies and Applications. 2022June;(3):100184en_US
dc.identifier.urihttps://doi.org/10.1016/j.carpta.2022.100184
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/11191
dc.publisherCarbohydrate Polymer Technologies and Applicationsen_US
dc.subjectBioink ; Printability; Cocultures; Dermal-Epidermal Construct; In vitro Tissue Engineeringen_US
dc.title. Exploring the Potential of Alginate-Gelatin-Diethylaminoethyl Cellulose Fibrinogen based Bioink for 3D Bioprinting of Skinen_US
dc.typeArticleen_US
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