A DOT-IMMUNOBINDING ASSAY FOR THE LABORATORY DIAGNOSIS OF TUBERCULOUS MENINGITIS AND ITS COMPARISON WITH ENZYME-LINKED-IMMUNOSORBENT-ASSAY

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dc.contributorRADHAKRISHNAN, VV
dc.contributorMATHAI, A
dc.date.accessioned2012-12-04T11:43:12Z
dc.date.available2012-12-04T11:43:12Z
dc.date.issued1991
dc.description.abstractIn an attempt to establish an alternative to standard bacteriological methods in the laboratory diagnosis of tuberculous meningitis (TBM), a simple dot-immunobinding assay (Dot-Iba) was standardized to detect Mycobacterium tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of patients with TBM. Sensitivity and specificity of Dot-Iba was compared with conventional enzyme-linked immunosorbent assay (ELISA) and standard bacteriological techniques. The Dot-Iba showed excellent correlation with indirect ELISA for the detection of antimycobacterial antibody in CSF and showed 60% sensitivity and 100% specificity in culture-negative patients with TBM. However Dot-Iba was less sensitive for the detection of antigen 5 in CSFs and showed false negative results (60%) in culture-positive patients with TBM.
dc.identifier.citationJOURNAL OF APPLIED BACTERIOLOGY. 71; 5; 428-433en_US
dc.identifier.urihttp://dx.doi.org/10.1111/j.1365-2672.1991.tb03812.x
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/1761436
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/17
dc.publisherJOURNAL OF APPLIED BACTERIOLOGY
dc.subjectLaboratory Diagnosis
dc.titleA DOT-IMMUNOBINDING ASSAY FOR THE LABORATORY DIAGNOSIS OF TUBERCULOUS MENINGITIS AND ITS COMPARISON WITH ENZYME-LINKED-IMMUNOSORBENT-ASSAY
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