Alginate stabilized gold nanoparticle as multidrug carrier: Evaluationof cellular interactions and hemolytic potential

Dey, S; Sherly, MCD; Rekha, MR; Sreenivasan, K

Alginate stabilized gold nanoparticle as multidrug carrier: Evaluationof cellular interactions and hemolytic potential

Date

2015-09

Authors

Publisher

Carbohydrate Polymers

Abstract

This work delineates the synthesis of curcumin (Ccm) and methotrexate (MTX) conjugated biopolymer stabilized AuNPs (MP@Alg–Ccm AuNPs). The dual drug conjugated nano-vector was characterized by FTIR, 1H NMR and UV–vis spectroscopic techniques. Hydrodynamic diameter and surface charge of the AuNPs were determined by DLS analysis and the spherical particles were visualized by TEM. MP@Alg–Ccm AuNPs exhibited improved cytotoxic potential against C6 glioma and MCF-7 cancer cell lines and was found to be highly hemocompatible. MP@Alg–Ccm AuNPs also exhibited active targeting efficiency against MCF-7 cancer cells due to the presence of “antifolate” drug MTX. Thus MP@Alg–Ccm AuNPs may find potential application in targeted combination chemotherapy for the treatment of cancer. The study is also interesting from the synthetic point of view because, here generation of AuNPs was done using “green chemical” alginate and dual drug conjugated AuNPs were created in two simple reaction steps using “green solvent” water

Keywords

Curcumin Alginate Polymer–drug conjugates Methotrexate Gold nanoparticles Confocal laser scanning microscopy

Citation

Dey S, Sherly MCD, Rekha MR and Sreenivasan K. Alginate stabilized gold nanoparticle as multidrug carrier: Evaluationof cellular interactions and hemolytic potential. Carbohydrate Polymers. 2016;136:71-80