Influence of matrix and bulk behaviour of an injectable hydrogel on the survival of encapsulated cardiac cells
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Date
2015
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RSC ADVANCES
Abstract
Cytocompatibility, suitable porosity, higher equilibrium water content and tissue like elasticity are the demanding criteria required to design a hydrogel for cell encapsulation and delivery. Here a mechanically stable cell supporting synthetic hydrogel was fabricated from poly(propylene fumarate-co-ethylene glycol)/PEGDA by redox initiating polymerisation for cell encapsulation. A hydrogel prepared with 93.5% poly(propylene fumarate-co-ethylene glycol) and 6.5% PEGDA acquired matrix and bulk characteristics of equilibrium water content (EWC) 84.45 + 0.80%, freezable water content 67.93%, Young modulus 212.2 +/- 0.02 kPa and pore diameter 88.64 +/- 18.96 mu m. This hydrogel with higher free water content, favourable pore dimensions and mechanical strength was used to encapsulate cardiomyoblasts. The encapsulated cardiomyoblasts were showing increasing viability from 3-30 days with viable green fluorescence. The matrix and bulk characteristics of the hydrogel are favourable and elicited uniform, green fluorescing, live cardiomyoblasts (H9c2) inside with 150% cell viability (MTT assay) and uniform ECM protein distribution after 30 days. The slow in vitro degradation of the hydrogel in physiological-like conditions is favourable for the delivery and retention of the encapsulated cells at the injection site.
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Chemistry
Citation
5 ,40;31439-31449