Evaluation of Antitumor Activity of Hesperetin-Loaded Nanoparticles Against DMBA-Induced Oral Carcinogenesis Based on Tissue Autofluorescence Spectroscopy and Multivariate Analysis
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Date
2015-06
Journal Title
Journal ISSN
Volume Title
Publisher
J Fluoresc
Abstract
The present study is designed to understand the
nature of endogenous fluorophores and cellular metabolism
that occur in the experimental oral carcinogenesis and to assess
their feasibility for antitumor efficacy of hesperetinloaded
nanoparticles (HETNPs) in comparison with native
hesperetin (HET) against 7,12-dimethyl benz(a) anthracene
(DMBA)-induced oral carcinogenesis using fluorescence
spectroscopy. The fluorescence emission spectra of the control
and the experimental buccal mucosa are recorded at an excitation
wavelength of 320 nm with an emission ranging from
350 to 550 nm. The results show that there is a reduced contribution
from the emission of collagen, nicotinamide adenine
dinucleotide (NADH) and flavin adenine dinucleotide (FAD),
inDMBA-induced tumor tissues as compared with the control
tissues. Furthermore, there was significant decrease in the optical
redox ratio [(FAD/ (NADH+FAD)] is observed in
DMBA-induced tumor tissues, which indicates an increased
metabolic activity when compared to the control tissues. Oral
administration of HET and its nanoparticulates restored the
status of endogenous fluorophores emission and would have
a higher redox ratio in the buccal mucosa of DMBA painted
animals. Taken together, the treatment of nanoparticulate
hesperetin was found to be more effective than native
hesperetin in improving the status of endogenous
fluorophores to a near normal range in DMBA-induced hamster
buccal pouch carcinogenesis. The results of this study
raise the important possibility that fluorescence spectroscopy
in conjunction with PC-LDA has tremendous potential for
monitor or potentially predict response to therapy
Description
Keywords
Endogenous fluorophores . Oral carcinogenesis . Nanoparticluate hesperetin . Multivariate analysis
Citation
Gurushankar K, Nazeer SS, Jayasree RS, Krishnakumar N.. Evaluation of Antitumor Activity of Hesperetin-Loaded Nanoparticles Against DMBA-Induced Oral Carcinogenesis Based on Tissue Autofluorescence Spectroscopy and Multivariate Analysis.. J Fluoresc. 2015.