Effects of double cross-linking technique on the enzymatic degradation and calcification of bovine pericardia

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dc.contributorVasudev, SC
dc.contributorChandy, T
dc.contributorSharma, CP
dc.contributorMohanty, M
dc.contributorUmasankar, PR
dc.date.accessioned2012-12-04T11:43:59Z
dc.date.available2012-12-04T11:43:59Z
dc.date.issued2000
dc.description.abstractThe strength, resorption rates, and biocompatibility of collagenous biomaterials are profoundly influenced by the method of cross-linking. The in vitro and in viuo calcification and enzymatic degradation of bovine pericardia (BP) after a series of surface modifications were studied as a function of exposure time. Collagenase degradations of modified BP were monitored by scanning electron microscopy and tensile strength measurements. Bovine pericardium was modified by a combination of different tissue fixatives such as glutaraldehyde (GA), carbodiimide (EDC), diisocyanate (HMDIC), and polyethylene glycol (PEG). GA-PEG-EDC-PEG and GA-PEG-HMDIC-PEG combination treated BP retained maximum stability in collagenase digestion compared to GATBP. In vitro calcification studies and in vivo rat subcutaneous implantations of modified pericardium have shown substantial reduction in the calcification of double cross-linked BP with PEG modification. Further, the biocompatibility aspects of pericardial tissues were established by platelet adhesion and octane contact angle. It seems that cross-links involving amino and carboxyl residues may provide new ways of controlling biodegradation and calcification.
dc.identifier.citationJOURNAL OF BIOMATERIALS APPLICATIONS. 14; 3; 273-295en_US
dc.identifier.urihttp://dx.doi.org/10.1106/HV9G-JEBF-Q7WU-P5XL
dc.identifier.urihttp://jba.sagepub.com/content/14/3/273.short
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/429
dc.publisherJOURNAL OF BIOMATERIALS APPLICATIONS
dc.subjectBiocompatibility
dc.titleEffects of double cross-linking technique on the enzymatic degradation and calcification of bovine pericardia
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