Metabolic Modulation by Medium-Chain Triglycerides Reduces Oxidative Stress and Ameliorates CD36-Mediated Cardiac Remodeling in Spontaneously Hypertensive Rat in the Initial and Established Stages of Hypertrophy
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Date
2017-03
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J Cardiac failure
Abstract
Background: Left ventricular hypertrophy (LVH) is characterized by a decrease in oxidation of longchain
fatty acids, possibly mediated by reduced expression of the cell-surface protein cluster of differentiation
36 (CD36). Spontaneously hypertensive rats (SHRs) were therefore supplemented with medium-chain triglycerides
(MCT), a substrate that bypasses CD36, based on the assumption that the metabolic modulation
will ameliorate ventricular remodeling.
Methods: The diet of 2-month-old and 6-month-old SHRs was supplemented with 5% MCT (Tricaprylin),
for 4 months. Metabolic modulation was assessed by mRNA expression of peroxisome proliferator–
activated receptor α and medium-chain acyl-CoA dehydrogenase. Blood pressure was measured noninvasively.
LVH was assessed with the use of hypertrophy index, cardiomyocyte cross-sectional area, mRNA expression
of B-type natriuretic peptide, cardiac fibrosis, and calcineurin-A levels. Oxidative stress indicators (cardiac
malondialdehyde, protein carbonyl, and 3-nitrotyrosine levels), myocardial energy level (ATP, phosphocreatine),
and lipid profile were determined.
Results: Supplementation of MCT stimulated fatty acid oxidation in animals of both age groups, reduced
hypertrophy and oxidative stress along with the maintenance of energy level. Blood pressure, body weight,
and lipid profile were unaffected by the treatment.
Conclusions: The results indicate that modulation of myocardial fatty acid metabolism by MCT prevents
progressive cardiac remodeling in SHRs, possibly by maintenance of energy level and decrease in oxidative
stress. (J Cardiac Fail 2017;23:240–251)
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Keywords
: Medium-chain triglyceride, Left ventricular hypertrophy, Spontaneously hypertensive rat, CD36, Oxidative stress
Citation
Ismael S, Lakshmi S, Remani K, Nair RR. Metabolic Modulation by Medium-Chain Triglycerides Reduces Oxidative Stress and Ameliorates CD36-Mediated Cardiac Remodeling in Spontaneously Hypertensive Rat in the Initial and Established Stages of Hypertrophy. J Cardiac failure. 2017;23(3):240-251