ApoB-INDEPENDENT ENZYME IMMUNOASSAY FOR LIPOPROTEIN(a) BY CAPTURE ON IMMOBILIZED LECTIN (JACALIN)
| dc.contributor.author | Sreekumar, A | |
| dc.contributor.author | Mandagini, G | |
| dc.contributor.author | Subramanian, SP | |
| dc.contributor.author | Sankunni, AP | |
| dc.date.accessioned | 2017-03-10T03:25:33Z | |
| dc.date.available | 2017-03-10T03:25:33Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 +/- 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV=6.4%). | |
| dc.identifier.citation | 34 ,2;166-179 | en_US |
| dc.identifier.uri | 10.1080/15321819.2012.699493 | |
| dc.identifier.uri | https://dspace.sctimst.ac.in/handle/123456789/9317 | |
| dc.publisher | JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | |
| dc.subject | Biochemistry & Molecular Biology; Immunology; Medical Laboratory Technology | |
| dc.title | ApoB-INDEPENDENT ENZYME IMMUNOASSAY FOR LIPOPROTEIN(a) BY CAPTURE ON IMMOBILIZED LECTIN (JACALIN) |