Use of chitosan as a biomaterial: Studies on its safety and hemostatic potential
No Thumbnail Available
Date
1997
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
Abstract
Chitosan, a mucopolysaccharide of marine origin, was studied for its safety and hemostatic potential. Its surface was treated with glutaraldehyde, carbodiimide, and plasma glow discharge to elicit effects of enzyme degradation. Of the seven enzymes used, leucine amino peptidase caused maximum degradation. Autoclaving appeared to be an ideal sterilizing method as it caused least decrease in tensile strength and effected a negligible rate of hemolysis. Sterilizing with glutaraldehyde with a physiologic pH retained the maximum tensile strength of chitosan. In vivo toxicity tests indicated that it is nontoxic, and the sterilized films were free of pyrogen. Coagulation and hemagglutination tests showed that the hemostatic mechanism of chitosan seems to be independent of the classical coagulation cascade and appears to be an interaction between the cell membrane of erythrocytes and chitosan. (C) 1997 John Wiley & Sons, Inc.
Description
Keywords
Biocompatibility
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. 34; 1; 21-28