In vitro binding of C-reactive protein on to chemically modified polymethyl methacrylate surfaces

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dc.contributor.authorSreenivasan, K
dc.contributor.authorRaj, V
dc.contributor.authorHan, PR
dc.date.accessioned2017-03-10T03:27:11Z
dc.date.available2017-03-10T03:27:11Z
dc.date.issued2007
dc.description.abstractPhosphorylethanolamine (PEA), C-reactive protein (CRP) specific ligand was coupled onto the surface of polymethyl methacrylate (PMMA) surface. Initially PMMA surface was modified by grafting glycidyl methacrylate (GMA). The anchoring of PEA on to PMMA was achieved through the ring opening reaction of epoxide group of GMA. The modified surface was characterized using FT-IR, scanning electron microscopy, energy dispersive X-ray analysis and contact angle measurement. The modified surface was found to bind CRP The adsorbed protein was visualized by staining Coomassie blue. The quantification of the extent of adsorption was carried out using spectrophotometric measurement. The methodology appears to be simple and expandable further for the development of a sensing element for CRP. (c) 2007 Elsevier B.V. All rights reserved.
dc.identifier.citation127 ,2;330-334en_US
dc.identifier.uri10.1016/j.snb.2007.04.026
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/9908
dc.publisherSENSORS AND ACTUATORS B-CHEMICAL
dc.subjectChemistry; Electrochemistry; Instruments & Instrumentation
dc.titleIn vitro binding of C-reactive protein on to chemically modified polymethyl methacrylate surfaces
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