Disulphide cross linked pullulan based cationic polymer for improved gene delivery and efflux pump inhibition

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dc.contributor.authorPriya, SS
dc.contributor.authorRekha, MR
dc.date.accessioned2017-03-10T03:26:18Z
dc.date.available2017-03-10T03:26:18Z
dc.date.issued2016
dc.description.abstractMultidrug resistance is a hurdle to successful cancer chemotherapy. Over expression of P-glycoprotein (P-gp) is a prime contributing factor for drug resistance. In this study, a disulphide cross-linked pullulan-based cationic polymer (PPSS) was synthesized to act simultaneously as gene delivery vehicle and efflux pump inhibitor. The PPSS nanoplexes were of size <200 nm with the zeta potential of +15 to +20 mV. The cytotoxicity studies using C6 and L929 cells showed that PPSS polymers are non-toxic even at high polymer concentrations. The PPSS/pDNA nanoplex showed superior uptake in confocal microscopy with 97% uptake by flow cytometry. The efficacy of efflux pump inhibition by the PPSS nanoplex was established by the enhanced intracellular retention of DOX. The enhanced cell death by p53/PPSS/DOX nanoplexes was attributed to the synergistic effect of P-gp inhibition and p53 transfection efficiency. Therefore, this multifunctional polymeric system may have significant promise for therapeutic application against cancer drug resistance. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier.citation146 ,;879-887en_US
dc.identifier.uri10.1016/j.colsurfb.2016.07.013
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/9600
dc.publisherCOLLOIDS AND SURFACES B-BIOINTERFACES
dc.subjectBiophysics; Chemistry; Materials Science
dc.titleDisulphide cross linked pullulan based cationic polymer for improved gene delivery and efflux pump inhibition
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