Over expression of cytosolic galectin-3 correlates with anti-apoptotic protein bcl-2: its significance in the differential diagnosis of follicular cell derived thyroid tumors in fnac
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Date
2016-02
Journal Title
Journal ISSN
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Publisher
International Journal of Current Research
Abstract
Fine Needle Aspiration Cytology is considered as a reliable and cost effective method for the initial evaluation of thyroid nodules. But it poses a diagnostic challenge in differentiating benign follicular adenoma from follicular carcinoma and follicular variant of papillary carcinoma as they have similar cytological appearance. Present study focused on the identification of an immuno marker for the differential diagnosis of thyroid malignancy and to compare the expression of Galectins and Bcl-2 to explain its role in apoptosis. We performed immunohistochemistry of galectin-1, galectin-3 and bcl-2 on cell block prepared from FNA material and corresponding tissue sections on 289 samples. Galectin-3 mRNA expression was quantified using Real time PCR. Western blotting was carried out in surgically excised fresh tissue specimens. Pap stained slides were also subjected to immunocytochemistry without de-staining. Galectin-3 was found to be highly significant (P<0.001) in the differential diagnosis of malignancy from adenoma with high diagnostic accuracy of 97.8%. All the samples of papillary carcinoma with distant metastasis showed intense expression of galectin-3 and bcl-2. A negative correlation in the expression pattern of galectin-3 and bcl-2 was observed in the case of thyroiditis. QRT- PCR revealed a significant over expression of galectin-3 (2.4 fold) in papillary carcinoma. The present study observed a correlation for the over expression of Galectin-3 with its anti-apoptotic activity and suggests galectin-3 immunohistochemistry along with morphological evaluation and quantitative assessment to eliminate unwanted thyroidectomies for benign nodules
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Keywords
Galectin-3, Galectin-1, Bcl-2, Fine needle aspiration cytology, Thyroid malignancy.
Citation
Shanone CP, Jissa VT, Shaji T, Preethi TR, Remani P, Sujathan K. Over expression of cytosolic galectin-3 correlates with anti-apoptotic protein bcl-2: its significance in the differential diagnosis of follicular cell derived thyroid tumors in fnac., International Journal of Current Research, 7, (10), 21780-21788