Nanoarchitectonics of fluorescent gold nanoclusters: A platform for image guided photodynamic therapy of hypoxic tumor

Metal nanoclusters are atomically precise materials comprising metal core of few atoms exhibiting unique photoluminescence properties, unlike their bigger counterparts. Some metal nanocluster with ligand-to-metal charge transfer, long-lived excited state and excited triplet state contribute to inherent photosensitizing (PS) property. However, the therapeutic efficacy of PDT is hindered by the insufficient oxygen supply (O2) in tumor microenvironment. In the present work, cysteine-capped gold nanocluster (AuC) are studied for their unique molecular architecture for PS efficiency. The co-existence of monodispersed and self-assembled structures contribute to the photoluminescence from the quantum confinement of electronic states and aggregation-induced emission (AIE) based PS property, respectively. In-silico model was performed to study the interaction of cysteine to gold cluster, its ground and excited-state properties and the charge transfer mechanism. The AuC as PS generates cytotoxic radicals in both Type I and Type II photodynamic pathways and the dominant radical species involved were elucidated by EPR spectroscopy. In vitro analysis in HeLa cells showed excellent biocompatibility and bioimaging properties. The intracellular ROS production and Live/Dead assay confirmed the generation of ROS in HeLa cells upon laser irradiation. The image-guided photodynamic property with synergistic Type I and Type II PDT reactions of AuC promises its potential application in cancer therapy in both hypoxic and normoxic conditions

Citation

Resmi.A.N, Sivaselvam S, Papasouli E, Kunnumpurathu J, Praveen CS, Koukaras EN, Rerat M, Karamanis P, Jayasree RS. Nanoarchitectonics of fluorescent gold nanoclusters: A platform for image guided photodynamic therapy of hypoxic tumor. Applied Materials Today. 2024 June 08