Suitability of measurement of swirling as a marker of platelet shape change in concentrates stored for transfusion

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dc.contributorMathai, Jaisy
dc.contributorResmi, K. R.
dc.contributorSulochana, P. V.
dc.contributorSathyabhama, S.
dc.contributorSaritha, G. Baby
dc.contributorKrishnan, Lissy K.
dc.date.accessioned2012-12-04T11:45:21Z
dc.date.available2012-12-04T11:45:21Z
dc.date.issued2006
dc.description.abstractPlatelet discoid shape is known to correlate with in vivo viability after transfusion. Measurement of shape change requires invasive sampling and laborious assays, which is difficult to perform in a blood transfusion center as a routine test for quality control of stored platelets. The objective of this study was to establish suitability of swirling assessment in stored platelet suspension as a routine test for quality check, by comparing platelet shape change measurement carried out in parallel. The study was done in two types of bags obtained from different manufactures (Groups 1 and 2). Platelet concentrates (PC) were stored for 120 h and samples drawn at 24-h intervals, optical analysis at 540nm was carried out to quantify shape change in response to an agonist adenosine diphosphate (ADP). The same bags were subjected to swirling assessment, by two blood bank personnel independently and graded as positive (+,++,+++) or as negative, based on the silky appearance of the suspension. Swirling negative platelets were prepared by storing platelets at 4 degrees C for 24 h and were compared with swirling positive platelets. Other parameters studied in the samples drawn at 24-h intervals were platelet count, mean platelet volume, and blood gases. Swirling assessment results correlated well with shape change measurement at each study period with a P value significant at 0.02 and 0.04 for group 1 and 2 bags, respectively. In the negative swirling controls, extent of shape change was lower than the extent in test bags, showing reduced capacity to respond to ADP at 4 degrees C. The results of the study indicate that by simple swirling measurements, stored PC can be monitored for loss of discoid shape before they are transfused. Gas tension and pH were with in permissible limits. Therefore, inspection of swirling can be a reliable method of quality control as it correlates with platelet function. The platelets that retain the discoid shape in vitro at the time of transfusion are expected to be functional in vivo.
dc.identifier.citationPLATELETS. 17; 6; 393-396en_US
dc.identifier.urihttp://dx.doi.org/10.1080/09537100600757695
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/16973500
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/1112
dc.publisherPLATELETS
dc.subjectHematology
dc.titleSuitability of measurement of swirling as a marker of platelet shape change in concentrates stored for transfusion
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