Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections

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dc.contributor.authorKumar, BS
dc.contributor.authorSandhyamani, S
dc.contributor.authorNazeer, SS
dc.contributor.authorJayasree, RS
dc.date.accessioned2017-03-10T03:28:22Z
dc.date.available2017-03-10T03:28:22Z
dc.date.issued2015
dc.description.abstractAutofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.
dc.identifier.citation52 ,1;107-110en_US
dc.identifier.urihttps://dspace.sctimst.ac.in/handle/123456789/10347
dc.publisherINDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
dc.subjectBiochemistry & Molecular Biology; Biophysics
dc.titleRapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections
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