A versatile approach for temporary storage and shipping of in vitro cultured cells, cell sheets and tissue engineered constructs – a preliminary report
| dc.contributor.author | Anju, MS | |
| dc.contributor.author | Athira, RK | |
| dc.contributor.author | Ramesh Babu, V | |
| dc.contributor.author | Anil Kumar, PR | |
| dc.contributor.author | Kasoju, N | |
| dc.date.accessioned | 2022-10-16T04:40:23Z | |
| dc.date.available | 2022-10-16T04:40:23Z | |
| dc.date.issued | 2022-09 | |
| dc.description.abstract | Temporary storage/ shipping of cell/ tissue engineering products from bench to bedside is a key aspect of regenerative medicine. The current proof-of-concept study presents a multipurpose device for temporary storage/ shipping of cell culture dishes containing cell/ tissue constructs. The device, made with readily available raw materials, contains three elements viz. a specialized lid, polymeric plates having grooves and a set of nuts and bolts. As part of the performance evaluation, the device was first subjected to a simulated storage/ shipping process, wherein the leak-proof and aseptic containment of the contents was demonstrated. Subsequently, the setup was used for temporary storage/ shipping of dishes having (a) L929 cell monolayers cultured on treated surfaces, (b) SIRC, HaCaT and A549 cell sheets cultured on thermo-responsive surfaces, (c) HOS-cell encapsulated agar gels and (d) HOS-cell seeded silk fibroin mats. The results showed that the health of cell monolayers/ cell sheets/ tissue constructs after the process was comparable to that before the process. The device was scalable, simple to handle, can be made for a single or multi-use purpose, and can be resizable to load other culture vessels. The design of the storage/ shipping device described in this report thus offers versatile features and applications. | en_US |
| dc.identifier.uri | https://doi.org/10.1016/j.engreg.2022.07.001 | |
| dc.identifier.uri | https://dspace.sctimst.ac.in/handle/123456789/11212 | |
| dc.publisher | Engineered Regeneration | en_US |
| dc.subject | Tissue engineeringScaffoldsTissue equivalentsTranslational medicine | en_US |
| dc.title | A versatile approach for temporary storage and shipping of in vitro cultured cells, cell sheets and tissue engineered constructs – a preliminary report | en_US |
| dc.type | Article | en_US |