Browsing by Author "Dey, S"

Now showing 1 - 7 of 7
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    Alginate stabilized gold nanoparticle as multidrug carrier: Evaluation of cellular interactions and hemolytic potential
    (CARBOHYDRATE POLYMERS, 2016) Dey, S; Sherly, MCD; Rekha, MR; Sreenivasan, K
    This work delineates the synthesis of curcumin (Ccm) and methotrexate (MTX) conjugated biopolymer stabilized AuNPs (MP@Alg-Ccm AuNPs). The dual drug conjugated nano-vector was characterized by FTIR, 1H NMR and UV-vis spectroscopic techniques. Hydrodynamic diameter and surface charge of the AuNPs were determined by DLS analysis and the spherical particles were visualized by TEM. MP@Alg-Ccm AuNPs exhibited improved cytotoxic potential against C6 glioma and MCF-7 cancer cell lines and was found to be highly hemocompatible. MP@Alg-Ccm AuNPs also exhibited active targeting efficiency against MCF-7 cancer cells due to the presence of "antifolate" drug MTX. Thus MP@Alg-Ccm AuNPs may find potential application in targeted combination chemotherapy for the treatment of cancer. The study is also interesting from the synthetic point of view because, here generation of AuNPs was done using "green chemical" alginate and dual drug conjugated AuNPs were created in two simple reaction steps using "green solvent" water. (C) 2015 Elsevier Ltd. All rights reserved.
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    Alginate stabilized gold nanoparticle as multidrug carrier: Evaluationof cellular interactions and hemolytic potential
    (Carbohydrate Polymers, 2015-09) Dey, S; Sherly, MCD; Rekha, MR; Sreenivasan, K
    This work delineates the synthesis of curcumin (Ccm) and methotrexate (MTX) conjugated biopolymer stabilized AuNPs (MP@Alg–Ccm AuNPs). The dual drug conjugated nano-vector was characterized by FTIR, 1H NMR and UV–vis spectroscopic techniques. Hydrodynamic diameter and surface charge of the AuNPs were determined by DLS analysis and the spherical particles were visualized by TEM. MP@Alg–Ccm AuNPs exhibited improved cytotoxic potential against C6 glioma and MCF-7 cancer cell lines and was found to be highly hemocompatible. MP@Alg–Ccm AuNPs also exhibited active targeting efficiency against MCF-7 cancer cells due to the presence of “antifolate” drug MTX. Thus MP@Alg–Ccm AuNPs may find potential application in targeted combination chemotherapy for the treatment of cancer. The study is also interesting from the synthetic point of view because, here generation of AuNPs was done using “green chemical” alginate and dual drug conjugated AuNPs were created in two simple reaction steps using “green solvent” water
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    Conjugating Curcumin to Water Soluble Polymer Stabilized Gold Nanoparticles via pH Responsive Succinate Linker
    (J. Mater. Chem B., 2015-01) Dey, S; Sreenivasan, K
    Curcumin is a natural product with immense medicinal assets. The low aqueous solubility and consequent poor bioavailability of curcumin are the most serious limitations to its utilization as a potential therapeutic agent. In order to enhance the aqueous solubility and bioavailability of the drug, we covalently conjugated curcumin onto the surface of gold nanoparticles (AuNPs) aided by a water soluble polymer via a succinate linker. Conjugation of curcumin was confirmed by fluorescence, FTIR, 1H NMR and UV-Visible spectroscopy and XRD studies. The size and surface charge of the AuNPs were determined by DLS, and the morphology was visualized by TEM. Aqueous solubility of curcumin was augmented upon conjugation with the polymer stabilized AuNPs. The pH responsive release of curcumin from the nano-vehicle ensures safer delivery of the drug at physiological pH. Cytotoxic potential and cellular uptake of curcumin conjugated AuNPs were assessed by MTT assay and fluorescence microscopy, respectively, using C6 glioma cancer cells. Thus, the curcumin conjugated polymer stabilized AuNPs circumvent limitations of curcumin and can find applications in pH responsive drug delivery.
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    Conjugation of curcumin onto alginate enhances aqueous solubility and stability of curcumin
    (Carbohydrate Polymers, 2015-01) Dey, S; Sreenivasan, K
    Curcumin is a potential drug for various diseases including cancer. Prime limitations associated with curcumin are low water solubility, rapid hydrolytic degradation and poor bioavailability. In order to redress these issues we developed Alginate-Curcumin (Alg-Ccm) conjugate which was characterized by FTIR and (1)H NMR spectroscopy. The conjugate self-assembled in aqueous solution forming micelles with an average hydrodynamic diameter of 459 ± 0.32 nm and negative zeta potential. The spherical micelles were visualized by TEM. The critical micelle concentration (CMC) of Alg-Ccm conjugate was determined. A significant enhancement in the aqueous solubility of curcumin was observed upon conjugation with alginate. Formation of micelles improved the stability of curcumin in water at physiological pH. The cytotoxic activity of Alg-Ccm was quantified by MTT assay using L-929 fibroblast cells and it was found to be potentially cytotoxic. Hence, Alg-Ccm could be a promising drug conjugate as well as a nanosized delivery vehicle.
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    Conjugation of curcumin onto alginate enhances aqueous solubility and stability of curcumin
    (Carbohydrate Polymers, 2013-09) Dey, S; Sreenivasan, K
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    Glutathione-bearing fluorescent polymer-curcumin conjugate enables simultaneous drug delivery and label-free cellular imaging
    (Polymer, 2015-09) Dey, S; Ambattu, LA; Hari, PR; Rekha, MR; Sreenivasan, K
    Curcumin is a natural polyphenol with potential anticancer property. However, the medicinal assets of curcumin cannot be properly utilized due to its low aqueous solubility, instability and subsequent poor bioavailability. Conjugation of curcumin to a suitable hydrophilic polymer is a useful approach for augmenting the aqueous solubility of the drug. In addition, nano-structured materials with intrinsic fluorescence property are of prime importance in the current biomedical domain. In this study, a novel glutathione containing biodegradable, non-toxic and water soluble, fluorescent polymer was developed. Curcumin was covalently conjugated to this polymer to fabricate nano-sized micelle forming fluorescent polymer-curcumin conjugate. The ability of the entity as a safer curcumin delivery vehicle and label-free cellular imaging probe was demonstrated in C6 glioma cells.
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    Synthesis, characterization, and cytotoxicity analysis of a biodegradable polyurethane
    (MATERIALS AND MANUFACTURING PROCESSES, 2006) Sarkar, S; Singha, PK; Dey, S; Mohanty, M; Adhikari, B
    A polyester urethane was synthesized for use in a biodegradable scaffold. The polyurethane was synthesized in a two-step process: first, ester diol was synthesized from lactic acid and polyethylene glycol 400 (PEG 400), then it was polymerized with toluene diisocyanate using dibutyl tin dilaurate (DBTDL) as a catalyst to form a polyester urethane. Polyester urethane has tensile strength of 51-59 MPa and elongation at fracture of 369-439%. FTIR and XRD were used to confirm the formation and structure of the polymer. Hydrolytic degradation was studied in different alkali solutions and in saline water. In order to assess the cellular response of this material, cytotoxicity analysis was carried out against the cell line.