Browsing by Author "Jibin, K"

Now showing 1 - 6 of 6
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    Asialoglycoprotein receptor targeted optical and magnetic resonance imaging and therapy of liver fbrosis using pullulan stabilized multi-functional iron oxide nanoprobe
    (Scientific Reports., 2021-09) Saraswathy, A; Nazeer, SS; Nimi, N; Hema, S; Parvathy, RS; Jibin, K; Victor, M; Fernandez, FB; Sabareeswaran, A; Shenoy, SJ; Harikrishna Varma, PR; Jayasre, RS
    Early diagnosis and therapy of liver fibrosis is of utmost importance, especially considering the increased incidence of alcoholic and non-alcoholic liver syndromes. In this work, a systematic study is reported to develop a dual function and biocompatible nanoprobe for liver specific diagnostic and therapeutic applications. A polysaccharide polymer, pullulan stabilized iron oxide nanoparticle (P-SPIONs) enabled high liver specificity via asialogycoprotein receptor mediation. Longitudinal and transverse magnetic relaxation rates of 2.15 and 146.91 mM−1 s−1 respectively and a size of 12 nm, confirmed the T2 weighted magnetic resonance imaging (MRI) efficacy of P-SPIONs. A current of 400A on 5 mg/ml of P-SPIONs raised the temperature above 50 °C, to facilitate effective hyperthermia. Finally, a NIR dye conjugation facilitated targeted dual imaging in liver fibrosis models, in vivo, with favourable histopathological results and recommends its use in early stage diagnosis using MRI and optical imaging, and subsequent therapy using hyperthermia.
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    Graphene−Gold NanohybridBased Surface-Enhanced Raman Scattering Platform on a Portable Easyto-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells
    (ACS Sustainable Chemistry and Engineering, 2021-11) Jibin, K; Ramesh Babu, V; Jayasree, RS
    Although, cancer is considered as a localized disease in its premature stages, in certain types of cells, it results in metastasis, which accounts for over 90% of the total cancer deaths in the world. During the metastatic stage, cancer cells migrate from the primary tumor site to secondary sites through the circulating bloodstream, resulting in metastasis at unexpected body parts. This situation makes it critical to identify and quantify the circulating tumor cells (CTCs) during the early stages of tumorigenesis. Despite the clinical importance and progress of liquid biopsy and CTC-based cancer diagnostics, it still remains extremely challenging to develop systems to detect CTCs in cancer patients as its presence is extremely low in number (1–10 CTCs per mL of blood plasma). In this article, we discuss about the design and fabrication of a robust and user-friendly custom-designed nanotag-enabled portable filter-based sensor system for the selective separation and isolation of circulating breast cancer cells from whole blood. The filter sensor platform basically consists of a centrifugal prototype to quickly transfer unprocessed blood samples up to 5 mL within 60 s, isolating the circulating breast cancer cells selectively over the anti-EpCAM antibody-immobilized polycarbonate (PC) filter. Moreover, it is also equipped with a sandwich complex of a surface-enhanced Raman scattering (SERS) nanotag comprising a gold–graphene hybrid integrated with anti-ErbB2 antibody (Au-rGO@anti-ErbB2) for the SERS-assisted quantification of CTCs. As compared to the immunoaffinity-based CTC isolation platforms, the centrifugal force-based SERS nanotag enabled filter sensor system proved to be a clog free, highly sensitive, and selective platform toward the rapid isolation of viable breast cancer cells up to 5 tumor cells/mL from whole blood without any sample pretreatment. The high sensitivity, ease of use, and quick availability of results recommends the current filter sensor platform as a universal liquid biopsy technique for the quantification of CTCs.
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    Nanohybrids of Magnetically Intercalated Optical Metamaterials for Magnetic Resonance/Raman Imaging and In Situ Chemodynamic/Photothermal Therapy
    (ACS Applied Biomaterials, 2021-07) Jibin, K; Victor, M; Saranya, G; Hema, S; Murali, V; Maiti, KK; Jayasree, RS
    Target-specific reactive oxygen species (ROS)-based cancer treatments with high therapeutic efficacy and minimal side effects have been identified recently as a potentially effective cancer management strategy. Herein, we report the fabrication of a targeted nanotheranostic agent built on an iron oxide nanoparticle-decorated graphene–gold hybrid [plasmonic magnetic nanoprobe (PMNP)] for self-guided magnetic resonance (MR)/surface-enhanced Raman scattering imaging and photothermal therapy (PTT)/chemodynamic therapy (CDT). In the presence of glutathione, which is abundant in the tumor environment, the iron oxide nanoparticles undergo in situ reduction, which in turn generates hydroxyl radicals via a Fenton reaction to realize targeted destruction of tumor cells. Moreover, the localized production of heat benefited from the near-infrared absorption of the PMNP accelerates the intratumoral ROS generation process, with a synergistic effect of CDT/PTT. Furthermore, the probe offers an accurate visualization of the intracellular localization of the material through SERS/MR dual imaging channels. In view of the advantages offered by the tumor-specific stimuli-responsive nature of the probe, the PMNP presents as an effective tool for cancer management.
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    Nanotechnology in cardiac stem cell therapy: cell modulation, imaging and gene delivery
    (RSC Advances. 2021, 2021-10) Sarathkumar, E; Marina, V; Menon, JA; Jibin, K; Suresh, P; Jayasree, RS
    The wide arena of applications opened by nanotechnology is multidimensional. It is already been proven that its prominence can continuously influence human life. The role of stem cells in curing degenerative diseases is another major area of research. Cardiovascular diseases are one of the major causes of death globally. Nanotechnology-assisted stem cell therapy could be used to tackle the challenges faced in the management of cardiovascular diseases. In spite of the positive indications and proven potential of stem cells to differentiate into cardiomyocytes for cardiac repair and regeneration during myocardial infarction, this therapeutic approach still remains in its infancy due to several factors such as non-specificity of injected cells, insignificant survival rate, and low cell retention. Attempts to improve stem cell therapy using nanoparticles have shown some interest among researchers. This review focuses on the major hurdles associated with cardiac stem cell therapy and the role of nanoparticles to overcome the major challenges in this field, including cell modulation, imaging, tracking and gene delivery.
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    Optically Controlled Hybrid Metamaterial of Plasmonic Spiky Gold Inbuilt Graphene Sheets for Bimodal Imaging Guided Multimodal Therapy
    (Journal of Biomaterials Science, 2020-05) Jibin, K; Prasad, J; Saranya, G; Shenoy, SJ; Maiti, KK; Jayasree, RS
    The development of multifunctional molecular diagnostic platforms for the concordant visualization and treatment of diseases with high sensitivity and resolution has recently become a crucial strategy in cancer management. Thus, engineering functional metamaterials with high therapeutic and imaging capabilities to elucidate diseases from their morphological behaviors to physiological mechanisms is an unmet need in the current scenario. Here, we report the design of a unique hybrid plasmonic nanoarchitecture for targeted multiple phototherapies of breast cancer by simultaneous real-time monitoring through fluorescence and surface-enhanced Raman scattering (SERS) techniques. The nanoframework consisted of plasmonic gold-graphene hybrids tethered with folic acid-ligated chitosan-modified photosensitizer (PpIX) to afford target-specific localized photothermal and photodynamic therapy. The hybrid vehicle also served as an excellent nanocarrier for the efficient loading and stimuli-responsive release of the chemotherapeutic drug doxorubicin (DOX) to enhance the therapeutic efficacy, thereby forming a trimodal nanomedicine against cancer. The cytotoxic effects induced by the cumulative action of the triplet therapeutic tools were visualized through both fluorescence and SERS imaging channels. Moreover, it also generated synchronized therapeutic effects resulting in the effective regression of tumor volume without propagating any toxic effects to other organs of the animals. Taken together, by virtue of strong light–matter interactions, the nanoprobe showed enhanced photoadsorption, which facilitated amplified light-reactive therapeutic and imaging efficacies along with targeted and enhanced chemotherapy, both in vitro and in vivo, which may offer promising outcomes in clinical research.