Browsing by Author "KRISHNAN, VVR"

Now showing 1 - 2 of 2
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    ENZYME-LINKED-IMMUNOSORBENT-ASSAY TO DETECT MYCOBACTERIUM-TUBERCULOSIS ANTIGEN-5 AND ANTIMYCOBACTERIAL ANTIBODY IN CEREBROSPINAL-FLUID OF PATIENTS WITH TUBERCULOUS MENINGITIS
    (JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1991)
    Inhibition ELISA and indirect ELISA was standardised to detect Mycobacterium tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of 75 patients with tuberculous meningitis, (TBM) and 75 patients with non-tuberculous neurological diseases (control group). In both ELISAs, no false-negative results were observed in 15 culture proven patients with TBM. Detection of M. tuberculosis antigen 5 is more sensitive than detection of antimycobacterial antibody. However, both ELISAs showed 100% specificity for tuberculous aetiology in culture-negative patients with TBM. ELISA should be considered as one of the approaches in the laboratory diagnosis of TBM, particularly when standard bacteriological methods are unable to demonstrate M. tuberculosis in CSF specimens of patients with TBM.
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    ISOLATION OF 2 ANTIGENS FROM THE CULTURE FILTRATES OF MYCOBACTERIUM-TUBERCULOSIS AND THEIR APPLICATIONS IN THE LABORATORY DIAGNOSIS OF THE TUBERCULOUS MENINGITIS
    (MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 1991)
    Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzyme-linked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than IAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.